Studies on Sulfhydryl Groups in Sera of Normal Humans1

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The determination of sulfhydryl groups in serum. I. Methods and results on normal sera.

Compounds containing sulfhydryl groups have occupied a unique position in metabolic and chemical studies because of their intimate association with the problems of protein structure (l), enzyme function (2), and cellular proliferation (3). Kolthoff and Harris (4) described an amperometric method for the determination of mercaptans which Benesch and Benesch adapted for use with amino acids and p...

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Sulfhydryl Groups in Proteins

Egg albumin in the native, unaltered state, does not give tests characteristic of sulfhydryl groups. When, however, this protein is treated in any one of several ways, such as by heat (8, 9, 17), ultraviolet irradiation (lo), shaking (lo), or by solution in urea or other amides (7), free sulfhydryl groups make their appearance. The amount of free -SH groups appearing in egg albumin through the ...

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Studies on some aspects of the rôle of sulfhydryl groups in morphogenesis.

THE importance of sulfhydryl compounds in the process of morphogenesis was pointed out by Brachet (1950). Using specific inhibitors of-SH such as chloroacetophenone, it has been shown that embryos develop a number of malformations when the activity of-SH groups is interfered with (Beatty, 1951; Deuchar, 1957; Lakshmi, 1962a). It has been observed that the abnormalities are mainly in the nervous...

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Sulfhydryl Groups in Films of Egg Albumin

1. The same number of SH groups reduces ferricyanide in surface films of egg albumin as in albumin denatured by urea, guanidine hydrochloride, Duponol, or heat, provided the ferricyanide reacts with films while they still are at the surface and with the denatured proteins while the denaturing agent (urea, heat, etc.) is present. 2. The SH groups of a suspension of egg albumin made by clumping t...

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The contractility of the thread model prepared from the KCl-soluble proteins of the egg and in vivo factors for the contraction are investigated in Hemicentrotus, Anthocidaris, and Pseudocentrotus eggs. The contractility of the thread model induced by metal ions or cystine changes during development in the characteristic pattern of high at the metaphase and low at the monaster and the interkine...

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ژورنال

عنوان ژورنال: Journal of Investigative Dermatology

سال: 1954

ISSN: 0022-202X

DOI: 10.1038/jid.1954.53